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mab2125 anti rat antibody  (R&D Systems)


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    R&D Systems mab2125 anti rat antibody
    Mab2125 Anti Rat Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mab2125+anti+rat+antibody/Mouse+LYVE-1+Antibody/pm41192429-673-16-14
    Average 94 stars, based on 48 article reviews
    mab2125 anti rat antibody - by Bioz Stars, 2026-11
    94/100 stars

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    Immunopeptidomics:

    Article Title: Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MHC-II-biotin (M5/114.15.2) ThermoFischer 13-5321-82 Rat anti-mouse Lyve-1 (ALY7) R&D Systems MAB2125 Anti-rat antibody coupled to Alexa488 Thermo Fisher Scientific A-11006 Streptavidin-Cy3 Jackson ImmunoResearch 016-160-084 Donkey Anti-Rat Cy3 AffiniPure IgG (H+L) Jackson ImmunoResearch 712-165-153 Hsp70 antibody (clone 5A5) Invitrogen MA3-007 Biological Samples C57BL/6J mice (6–12 weeks) The Jackson Laboratory Strain number: 000664 Zebrafish: Tg(mpx:GFP)i114 Schaaf Lab / ZFIN ZDB-ALT-060623-3 Zebrafish: Tg(mpeg1:mCherry)umsF001 Schaaf Lab / ZFIN ZDB-ALT-141023-1 Chemicals, Peptides, and Recombinant Proteins RPMI 1640 medium Thermo Fisher Scientific 11875093 L-glutamine Thermo Fisher Scientific 25030081 Fetal bovine serum Gibco A5256701 Penicillin/Streptomycin Thermo Fisher Scientific 15140122 DMEM PAN Biotech P04-05550 DMSO Sigma-Aldrich D2650 β-Mercaptoethanol Gibco 11528926 GM-CSF (hybridoma supernatant) In-house N/A Lipopolysaccharide (LPS) Sigma-Aldrich L2630 Paraformaldehyde (PFA), 4% Electron Microscopy Sciences 15710 Triton X-100 Sigma-Aldrich T8787 Bovine serum albumin (BSA) Sigma-Aldrich A9647 Tricaine Sigma-Aldrich A5040 Beclomethasone Sigma-Aldrich B9771 CellROX Green Invitrogen C10444 RIPA buffer Abcam ab156034 cOmplete protease inhibitor Roche 12352200 Anti-phosphatase cocktail Abcam ab201112 Calbryte 520 AAT Bioquest 20650 HBSS++ Sigma-Aldrich H6648 Ultra-Pure Agarose Invitrogen 16500100 Phenol red-free RPMI Gibco 11835030 Ascorbic acid Sigma-Aldrich A7506 CCL19 R&D Systems 361-MI PDMS (silicon rubber + curing agent) Dow Corning Sylgard 184 Perfluorooctyltrichlorosilane Sigma-Aldrich 448931-10G Dextran Sigma-Aldrich D1037 Blebbistatin Sigma-Aldrich B0560 CK666 Sigma-Aldrich SML0006 α-Amanitin Sigma-Aldrich A2263 17-AAG (Tanespimycin) Sigma-Aldrich A8476 PLL-PEG SuSoS AG PLL(20)-g[3.5]-PEG(2) PureCol collagen Advanced BioMatrix 5005 MEM 10x Sigma-Aldrich M0275 (Continued on next page) Developmental Cell 61, 1–16.e1–e12, February 11, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaHCO3 (7.5%) Thermo Fisher 25080094 Aqua regia (HNO3 + HCl) In-house preparation N/A Piranha solution (H2SO4 + H2O2) In-house preparation N/A APTES Sigma-Aldrich A3648 Fibronectin Sigma-Aldrich F0895 N-Formyl-Met-Leu-Phe Sigma-Aldrich F3506 DAPI Invitrogen D1306 NucBlue Invitrogen R37605 Critical Commercial Assays Pierce BCA protein assay kit Thermo Scientific 23227 Deposited Data Custom-made code for analysis GitHub https://github.com/CompanyGarrido/ thermo_migration Experimental Models: Cell Lines

    Recombinant:

    Article Title: Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MHC-II-biotin (M5/114.15.2) ThermoFischer 13-5321-82 Rat anti-mouse Lyve-1 (ALY7) R&D Systems MAB2125 Anti-rat antibody coupled to Alexa488 Thermo Fisher Scientific A-11006 Streptavidin-Cy3 Jackson ImmunoResearch 016-160-084 Donkey Anti-Rat Cy3 AffiniPure IgG (H+L) Jackson ImmunoResearch 712-165-153 Hsp70 antibody (clone 5A5) Invitrogen MA3-007 Biological Samples C57BL/6J mice (6–12 weeks) The Jackson Laboratory Strain number: 000664 Zebrafish: Tg(mpx:GFP)i114 Schaaf Lab / ZFIN ZDB-ALT-060623-3 Zebrafish: Tg(mpeg1:mCherry)umsF001 Schaaf Lab / ZFIN ZDB-ALT-141023-1 Chemicals, Peptides, and Recombinant Proteins RPMI 1640 medium Thermo Fisher Scientific 11875093 L-glutamine Thermo Fisher Scientific 25030081 Fetal bovine serum Gibco A5256701 Penicillin/Streptomycin Thermo Fisher Scientific 15140122 DMEM PAN Biotech P04-05550 DMSO Sigma-Aldrich D2650 β-Mercaptoethanol Gibco 11528926 GM-CSF (hybridoma supernatant) In-house N/A Lipopolysaccharide (LPS) Sigma-Aldrich L2630 Paraformaldehyde (PFA), 4% Electron Microscopy Sciences 15710 Triton X-100 Sigma-Aldrich T8787 Bovine serum albumin (BSA) Sigma-Aldrich A9647 Tricaine Sigma-Aldrich A5040 Beclomethasone Sigma-Aldrich B9771 CellROX Green Invitrogen C10444 RIPA buffer Abcam ab156034 cOmplete protease inhibitor Roche 12352200 Anti-phosphatase cocktail Abcam ab201112 Calbryte 520 AAT Bioquest 20650 HBSS++ Sigma-Aldrich H6648 Ultra-Pure Agarose Invitrogen 16500100 Phenol red-free RPMI Gibco 11835030 Ascorbic acid Sigma-Aldrich A7506 CCL19 R&D Systems 361-MI PDMS (silicon rubber + curing agent) Dow Corning Sylgard 184 Perfluorooctyltrichlorosilane Sigma-Aldrich 448931-10G Dextran Sigma-Aldrich D1037 Blebbistatin Sigma-Aldrich B0560 CK666 Sigma-Aldrich SML0006 α-Amanitin Sigma-Aldrich A2263 17-AAG (Tanespimycin) Sigma-Aldrich A8476 PLL-PEG SuSoS AG PLL(20)-g[3.5]-PEG(2) PureCol collagen Advanced BioMatrix 5005 MEM 10x Sigma-Aldrich M0275 (Continued on next page) Developmental Cell 61, 1–16.e1–e12, February 11, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaHCO3 (7.5%) Thermo Fisher 25080094 Aqua regia (HNO3 + HCl) In-house preparation N/A Piranha solution (H2SO4 + H2O2) In-house preparation N/A APTES Sigma-Aldrich A3648 Fibronectin Sigma-Aldrich F0895 N-Formyl-Met-Leu-Phe Sigma-Aldrich F3506 DAPI Invitrogen D1306 NucBlue Invitrogen R37605 Critical Commercial Assays Pierce BCA protein assay kit Thermo Scientific 23227 Deposited Data Custom-made code for analysis GitHub https://github.com/CompanyGarrido/ thermo_migration Experimental Models: Cell Lines

    Electron Microscopy:

    Article Title: Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MHC-II-biotin (M5/114.15.2) ThermoFischer 13-5321-82 Rat anti-mouse Lyve-1 (ALY7) R&D Systems MAB2125 Anti-rat antibody coupled to Alexa488 Thermo Fisher Scientific A-11006 Streptavidin-Cy3 Jackson ImmunoResearch 016-160-084 Donkey Anti-Rat Cy3 AffiniPure IgG (H+L) Jackson ImmunoResearch 712-165-153 Hsp70 antibody (clone 5A5) Invitrogen MA3-007 Biological Samples C57BL/6J mice (6–12 weeks) The Jackson Laboratory Strain number: 000664 Zebrafish: Tg(mpx:GFP)i114 Schaaf Lab / ZFIN ZDB-ALT-060623-3 Zebrafish: Tg(mpeg1:mCherry)umsF001 Schaaf Lab / ZFIN ZDB-ALT-141023-1 Chemicals, Peptides, and Recombinant Proteins RPMI 1640 medium Thermo Fisher Scientific 11875093 L-glutamine Thermo Fisher Scientific 25030081 Fetal bovine serum Gibco A5256701 Penicillin/Streptomycin Thermo Fisher Scientific 15140122 DMEM PAN Biotech P04-05550 DMSO Sigma-Aldrich D2650 β-Mercaptoethanol Gibco 11528926 GM-CSF (hybridoma supernatant) In-house N/A Lipopolysaccharide (LPS) Sigma-Aldrich L2630 Paraformaldehyde (PFA), 4% Electron Microscopy Sciences 15710 Triton X-100 Sigma-Aldrich T8787 Bovine serum albumin (BSA) Sigma-Aldrich A9647 Tricaine Sigma-Aldrich A5040 Beclomethasone Sigma-Aldrich B9771 CellROX Green Invitrogen C10444 RIPA buffer Abcam ab156034 cOmplete protease inhibitor Roche 12352200 Anti-phosphatase cocktail Abcam ab201112 Calbryte 520 AAT Bioquest 20650 HBSS++ Sigma-Aldrich H6648 Ultra-Pure Agarose Invitrogen 16500100 Phenol red-free RPMI Gibco 11835030 Ascorbic acid Sigma-Aldrich A7506 CCL19 R&D Systems 361-MI PDMS (silicon rubber + curing agent) Dow Corning Sylgard 184 Perfluorooctyltrichlorosilane Sigma-Aldrich 448931-10G Dextran Sigma-Aldrich D1037 Blebbistatin Sigma-Aldrich B0560 CK666 Sigma-Aldrich SML0006 α-Amanitin Sigma-Aldrich A2263 17-AAG (Tanespimycin) Sigma-Aldrich A8476 PLL-PEG SuSoS AG PLL(20)-g[3.5]-PEG(2) PureCol collagen Advanced BioMatrix 5005 MEM 10x Sigma-Aldrich M0275 (Continued on next page) Developmental Cell 61, 1–16.e1–e12, February 11, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaHCO3 (7.5%) Thermo Fisher 25080094 Aqua regia (HNO3 + HCl) In-house preparation N/A Piranha solution (H2SO4 + H2O2) In-house preparation N/A APTES Sigma-Aldrich A3648 Fibronectin Sigma-Aldrich F0895 N-Formyl-Met-Leu-Phe Sigma-Aldrich F3506 DAPI Invitrogen D1306 NucBlue Invitrogen R37605 Critical Commercial Assays Pierce BCA protein assay kit Thermo Scientific 23227 Deposited Data Custom-made code for analysis GitHub https://github.com/CompanyGarrido/ thermo_migration Experimental Models: Cell Lines

    Protease Inhibitor:

    Article Title: Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies MHC-II-biotin (M5/114.15.2) ThermoFischer 13-5321-82 Rat anti-mouse Lyve-1 (ALY7) R&D Systems MAB2125 Anti-rat antibody coupled to Alexa488 Thermo Fisher Scientific A-11006 Streptavidin-Cy3 Jackson ImmunoResearch 016-160-084 Donkey Anti-Rat Cy3 AffiniPure IgG (H+L) Jackson ImmunoResearch 712-165-153 Hsp70 antibody (clone 5A5) Invitrogen MA3-007 Biological Samples C57BL/6J mice (6–12 weeks) The Jackson Laboratory Strain number: 000664 Zebrafish: Tg(mpx:GFP)i114 Schaaf Lab / ZFIN ZDB-ALT-060623-3 Zebrafish: Tg(mpeg1:mCherry)umsF001 Schaaf Lab / ZFIN ZDB-ALT-141023-1 Chemicals, Peptides, and Recombinant Proteins RPMI 1640 medium Thermo Fisher Scientific 11875093 L-glutamine Thermo Fisher Scientific 25030081 Fetal bovine serum Gibco A5256701 Penicillin/Streptomycin Thermo Fisher Scientific 15140122 DMEM PAN Biotech P04-05550 DMSO Sigma-Aldrich D2650 β-Mercaptoethanol Gibco 11528926 GM-CSF (hybridoma supernatant) In-house N/A Lipopolysaccharide (LPS) Sigma-Aldrich L2630 Paraformaldehyde (PFA), 4% Electron Microscopy Sciences 15710 Triton X-100 Sigma-Aldrich T8787 Bovine serum albumin (BSA) Sigma-Aldrich A9647 Tricaine Sigma-Aldrich A5040 Beclomethasone Sigma-Aldrich B9771 CellROX Green Invitrogen C10444 RIPA buffer Abcam ab156034 cOmplete protease inhibitor Roche 12352200 Anti-phosphatase cocktail Abcam ab201112 Calbryte 520 AAT Bioquest 20650 HBSS++ Sigma-Aldrich H6648 Ultra-Pure Agarose Invitrogen 16500100 Phenol red-free RPMI Gibco 11835030 Ascorbic acid Sigma-Aldrich A7506 CCL19 R&D Systems 361-MI PDMS (silicon rubber + curing agent) Dow Corning Sylgard 184 Perfluorooctyltrichlorosilane Sigma-Aldrich 448931-10G Dextran Sigma-Aldrich D1037 Blebbistatin Sigma-Aldrich B0560 CK666 Sigma-Aldrich SML0006 α-Amanitin Sigma-Aldrich A2263 17-AAG (Tanespimycin) Sigma-Aldrich A8476 PLL-PEG SuSoS AG PLL(20)-g[3.5]-PEG(2) PureCol collagen Advanced BioMatrix 5005 MEM 10x Sigma-Aldrich M0275 (Continued on next page) Developmental Cell 61, 1–16.e1–e12, February 11, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaHCO3 (7.5%) Thermo Fisher 25080094 Aqua regia (HNO3 + HCl) In-house preparation N/A Piranha solution (H2SO4 + H2O2) In-house preparation N/A APTES Sigma-Aldrich A3648 Fibronectin Sigma-Aldrich F0895 N-Formyl-Met-Leu-Phe Sigma-Aldrich F3506 DAPI Invitrogen D1306 NucBlue Invitrogen R37605 Critical Commercial Assays Pierce BCA protein assay kit Thermo Scientific 23227 Deposited Data Custom-made code for analysis GitHub https://github.com/CompanyGarrido/ thermo_migration Experimental Models: Cell Lines



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    Image Search Results


    Fig. 1 | Junctional heterogeneity in capillary LECs. a, Whole-mount immunofluorescence of ear skin from a 25-week-old Cdh5-GFP mouse expressing VE-cadherin-GFP fusion protein (VE-cad). Boxed areas magnified below show unsegmented (arrows) and focal (arrowheads) VE-cadherin+ junctions in lymphatic capillary (left) and precollecting vessel (middle), and continuous zipper junctions in LYVE1− collecting vessel (right). b, Immunofluorescence in 12-week-old mouse ear skin showing VE-cadherin colocalization with CLDN5 at junctions. Line intensity profiles through lines 1 and 2 of respective stainings are depicted. c, Immunofluorescence at the indicated ages depicting junctional heterogeneity. Boxed areas are magnified. d,e, Quantification of lymphatic vessel sprouting (percentage of spiky ends of all lymphatic capillary ends, n = 7, 12 per respective stage, mean ± s.e.m.; d) and LEC proliferation (percentage Ki67+ of all LECs by flow cytometry, n = 3, 6, 8, 6 mice per respective stage, mean ± s.e.m.; e). f, Whole-mount silver nitrate (Ag) staining of ear dermis showing deposits around cell perimeter, including the lobe tips (arrow),

    Journal: Nature

    Article Title: Dynamic cytoskeletal regulation of cell shape supports resilience of lymphatic endothelium.

    doi: 10.1038/s41586-025-08724-6

    Figure Lengend Snippet: Fig. 1 | Junctional heterogeneity in capillary LECs. a, Whole-mount immunofluorescence of ear skin from a 25-week-old Cdh5-GFP mouse expressing VE-cadherin-GFP fusion protein (VE-cad). Boxed areas magnified below show unsegmented (arrows) and focal (arrowheads) VE-cadherin+ junctions in lymphatic capillary (left) and precollecting vessel (middle), and continuous zipper junctions in LYVE1− collecting vessel (right). b, Immunofluorescence in 12-week-old mouse ear skin showing VE-cadherin colocalization with CLDN5 at junctions. Line intensity profiles through lines 1 and 2 of respective stainings are depicted. c, Immunofluorescence at the indicated ages depicting junctional heterogeneity. Boxed areas are magnified. d,e, Quantification of lymphatic vessel sprouting (percentage of spiky ends of all lymphatic capillary ends, n = 7, 12 per respective stage, mean ± s.e.m.; d) and LEC proliferation (percentage Ki67+ of all LECs by flow cytometry, n = 3, 6, 8, 6 mice per respective stage, mean ± s.e.m.; e). f, Whole-mount silver nitrate (Ag) staining of ear dermis showing deposits around cell perimeter, including the lobe tips (arrow),

    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for whole mount immunofluorescence (dilution 1:100-1:500): chicken anti-GFP (ab13970, Abcam), goat anti-mouse VEGFR3 (AF743, R&D Systems), goat anti-mouse VE-cadherin (R&D Systems, AF1002), goat anti-mouse PECAM1 (R&D, Systems AF3628), mouse anti-HA tag, Alexa Fluor 647 (Cell Signalling Technology, 6E2), rabbit anti-alpha tubulin (Abcam, ab52866), rabbit anti-GFP (A11122, Thermo Fisher Scientific), rabbit anti-DsRed (Takara Bio, 632496), rabbit anti-mouse LYVE1 (Reliatech, 103-PA50AG), rabbit anti-mouse CLDN5 (Invitrogen, 34-1600), rat anti-mouse PECAM1 (553370, BD Pharmingen), rat anti-mouse LYVE1-Alexa FluorTM 488, Clone ALY7 (Invitrogen, 53-0443-82), rat anti-mouse LYVE1 (R&D Systems (MAB2125), rat Anti-Mouse CD29 Clone 9EG7 (BD Pharmingen, 553715) All secondary antibodies were conjugated to Cy3(JIR, 712-165-153),(JIR, 711-166-152), Dylight 405 (JIR, 712-475-153), Alexa Fluor 488 (JIR, 703-545-155), (JIR, 712-545-153), (JIR, 711-545-152), Alexa Fluor 594 (JIR, 705-585-147), Alexa Fluor 647(JIR, 712-605-153),(JIR, 705-605-147),(JIR, 711-605-152) or Alexa Fluor 680 (JIR, 705-625-147) were raised in donkey and obtained from Jackson ImmunoResearch(JIR) .

    Techniques: Immunofluorescence, Expressing, Flow Cytometry, Staining

    Fig. 2 | Morphology and remodelling of intercellular overlaps between capillary LECs. a, Constructs for mosaic multicolour labelling of LECs using membrane-localized fluorescent proteins. b, Whole-mount immunofluorescence of mosaically labelled dermal LECs in a 6-week-old iMb2-Mosaic;Vegfr3-creERT2 mouse after 4-OHT treatment at 3 weeks, showing lobate shape in LYVE1+ capillaries and elongated shape in LYVE1− collectors (arrowheads). c, Whole-mount immunofluorescence of embryonic back skin (E17) or ear skin at indicated postnatal stages in iMb2-Mosaic;Vegfr3-creERT2 mice. d–f, Dermal LEC and vessel parameters, represented as mean ± s.d.: cell size (d, n = 24, 17 and 20 cells per respective stage), lobe number (e, n = 24, 48 and 20 cells per respective stage) and average lymphatic capillary width (f, n = 7, 5 and 9 mice per respective stage). Ordinary one-way ANOVA. g, Immunofluorescence of ear skin of a 12-week-old iMb2-Mosaic;Vegfr3-creERT2 mouse showing LYVE1 at LEC overlaps, with corresponding intensity plot. h, Double staining for cell surface and total LYVE1 (left), or with intradermally

    Journal: Nature

    Article Title: Dynamic cytoskeletal regulation of cell shape supports resilience of lymphatic endothelium.

    doi: 10.1038/s41586-025-08724-6

    Figure Lengend Snippet: Fig. 2 | Morphology and remodelling of intercellular overlaps between capillary LECs. a, Constructs for mosaic multicolour labelling of LECs using membrane-localized fluorescent proteins. b, Whole-mount immunofluorescence of mosaically labelled dermal LECs in a 6-week-old iMb2-Mosaic;Vegfr3-creERT2 mouse after 4-OHT treatment at 3 weeks, showing lobate shape in LYVE1+ capillaries and elongated shape in LYVE1− collectors (arrowheads). c, Whole-mount immunofluorescence of embryonic back skin (E17) or ear skin at indicated postnatal stages in iMb2-Mosaic;Vegfr3-creERT2 mice. d–f, Dermal LEC and vessel parameters, represented as mean ± s.d.: cell size (d, n = 24, 17 and 20 cells per respective stage), lobe number (e, n = 24, 48 and 20 cells per respective stage) and average lymphatic capillary width (f, n = 7, 5 and 9 mice per respective stage). Ordinary one-way ANOVA. g, Immunofluorescence of ear skin of a 12-week-old iMb2-Mosaic;Vegfr3-creERT2 mouse showing LYVE1 at LEC overlaps, with corresponding intensity plot. h, Double staining for cell surface and total LYVE1 (left), or with intradermally

    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for whole mount immunofluorescence (dilution 1:100-1:500): chicken anti-GFP (ab13970, Abcam), goat anti-mouse VEGFR3 (AF743, R&D Systems), goat anti-mouse VE-cadherin (R&D Systems, AF1002), goat anti-mouse PECAM1 (R&D, Systems AF3628), mouse anti-HA tag, Alexa Fluor 647 (Cell Signalling Technology, 6E2), rabbit anti-alpha tubulin (Abcam, ab52866), rabbit anti-GFP (A11122, Thermo Fisher Scientific), rabbit anti-DsRed (Takara Bio, 632496), rabbit anti-mouse LYVE1 (Reliatech, 103-PA50AG), rabbit anti-mouse CLDN5 (Invitrogen, 34-1600), rat anti-mouse PECAM1 (553370, BD Pharmingen), rat anti-mouse LYVE1-Alexa FluorTM 488, Clone ALY7 (Invitrogen, 53-0443-82), rat anti-mouse LYVE1 (R&D Systems (MAB2125), rat Anti-Mouse CD29 Clone 9EG7 (BD Pharmingen, 553715) All secondary antibodies were conjugated to Cy3(JIR, 712-165-153),(JIR, 711-166-152), Dylight 405 (JIR, 712-475-153), Alexa Fluor 488 (JIR, 703-545-155), (JIR, 712-545-153), (JIR, 711-545-152), Alexa Fluor 594 (JIR, 705-585-147), Alexa Fluor 647(JIR, 712-605-153),(JIR, 705-605-147),(JIR, 711-605-152) or Alexa Fluor 680 (JIR, 705-625-147) were raised in donkey and obtained from Jackson ImmunoResearch(JIR) .

    Techniques: Construct, Membrane, Immunofluorescence, Double Staining

    Fig. 3 | Cytoskeletal organization in lobate capillary LECs. a, Dot plot showing differential expression of cytoskeletal genes between capillary and collecting vessel LECs. Dot size illustrates percentage of cells with transcript counts, colour illustrates average expression (log2-fold difference). Cap, lymphatic capillary; Col, collecting vessel. b, Whole-mount immunofluorescence of adult ear skin showing microtubule network in dermal capillary LECs. Cell outline, based on VE-cadherin and LYVE1 staining, in red. c, Quantification of microtubule (MT) anchoring and density in capillary LECs in 9–12-week-old mice. Cell outline from c in green, with MT endpoints shown by yellow (concave) and purple (convex) dots. Data represent the percentage of MT anchoring (left; n = 5 LECs from five mice, 20–53 MT per cell), or MTs per µm of cortex in concave (right; n = 156 MTs, 5 LECs from five mice) versus convex (n = 56 MTs, 5 LECs

    Journal: Nature

    Article Title: Dynamic cytoskeletal regulation of cell shape supports resilience of lymphatic endothelium.

    doi: 10.1038/s41586-025-08724-6

    Figure Lengend Snippet: Fig. 3 | Cytoskeletal organization in lobate capillary LECs. a, Dot plot showing differential expression of cytoskeletal genes between capillary and collecting vessel LECs. Dot size illustrates percentage of cells with transcript counts, colour illustrates average expression (log2-fold difference). Cap, lymphatic capillary; Col, collecting vessel. b, Whole-mount immunofluorescence of adult ear skin showing microtubule network in dermal capillary LECs. Cell outline, based on VE-cadherin and LYVE1 staining, in red. c, Quantification of microtubule (MT) anchoring and density in capillary LECs in 9–12-week-old mice. Cell outline from c in green, with MT endpoints shown by yellow (concave) and purple (convex) dots. Data represent the percentage of MT anchoring (left; n = 5 LECs from five mice, 20–53 MT per cell), or MTs per µm of cortex in concave (right; n = 156 MTs, 5 LECs from five mice) versus convex (n = 56 MTs, 5 LECs

    Article Snippet: Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were used for whole mount immunofluorescence (dilution 1:100-1:500): chicken anti-GFP (ab13970, Abcam), goat anti-mouse VEGFR3 (AF743, R&D Systems), goat anti-mouse VE-cadherin (R&D Systems, AF1002), goat anti-mouse PECAM1 (R&D, Systems AF3628), mouse anti-HA tag, Alexa Fluor 647 (Cell Signalling Technology, 6E2), rabbit anti-alpha tubulin (Abcam, ab52866), rabbit anti-GFP (A11122, Thermo Fisher Scientific), rabbit anti-DsRed (Takara Bio, 632496), rabbit anti-mouse LYVE1 (Reliatech, 103-PA50AG), rabbit anti-mouse CLDN5 (Invitrogen, 34-1600), rat anti-mouse PECAM1 (553370, BD Pharmingen), rat anti-mouse LYVE1-Alexa FluorTM 488, Clone ALY7 (Invitrogen, 53-0443-82), rat anti-mouse LYVE1 (R&D Systems (MAB2125), rat Anti-Mouse CD29 Clone 9EG7 (BD Pharmingen, 553715) All secondary antibodies were conjugated to Cy3(JIR, 712-165-153),(JIR, 711-166-152), Dylight 405 (JIR, 712-475-153), Alexa Fluor 488 (JIR, 703-545-155), (JIR, 712-545-153), (JIR, 711-545-152), Alexa Fluor 594 (JIR, 705-585-147), Alexa Fluor 647(JIR, 712-605-153),(JIR, 705-605-147),(JIR, 711-605-152) or Alexa Fluor 680 (JIR, 705-625-147) were raised in donkey and obtained from Jackson ImmunoResearch(JIR) .

    Techniques: Quantitative Proteomics, Expressing, Immunofluorescence, Staining